Difference between revisions of "Inoculating Plates and Slants"

Revision as of 03:03, 22 October 2008

To steak yeast onto a plate you'll need:

a flame source for sterilizing the inoculation needle
an inoculation needle. A piece of wire works as well, but inoculation needles are cheap and you should pick one up when buying the petri dishes
a sample of yeast. This could be an empty Wyeast smack pack, Whitle Labs vial, dregs from a bottle conditioned beer, an active fermentation, yeast sediment .... In this case I had an old White Labs vial into which I filled sterile wort to get the yeast fermenting again before taking a sample.
an petri dish with malt agar that has been prepared in advance. (See [Making Plates Slants])

@@ Line 1: / Line 1: @@
 [[Image:Sreaking_needle.jpg]]
+===What's needed===
-[[Image:Streaking tools.jpg]]
+[[Image:Streaking tools.jpg|right]]
-[[Image:Streaking prep.jpg1]]
+To steak yeast onto a plate you'll need:
+* a flame source for sterilizing the inoculation needle
+* an inoculation needle. A piece of wire works as well, but inoculation needles are cheap and you should pick one up when buying the petri dishes
+* a sample of yeast. This could be an empty Wyeast smack pack, Whitle Labs vial, dregs from a bottle conditioned beer, an active fermentation, yeast sediment .... In this case I had an old White Labs vial into which I filled sterile wort to get the yeast fermenting again before taking a sample.
+* an petri dish with malt agar that has been prepared in advance. (See [Making Plates Slants])
+<div style="clear:both;"></div>
+[[Image:Streaking prep.jpg]]
+<div style="clear:both;"></div>
 [[Image:Streaking sampling.jpg]]
+<div style="clear:both;"></div>
 [[Image:Streaking streaking.jpg]]
+<div style="clear:both;"></div>
 [[Image:Streaking grown plate.jpg]]