Difference between revisions of "Inoculating Plates and Slants"
From German brewing and more
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[[Image:Sreaking_needle.jpg]] | [[Image:Sreaking_needle.jpg]] | ||
+ | ===What's needed=== | ||
− | [[Image:Streaking tools.jpg]] | + | [[Image:Streaking tools.jpg|right]] |
− | [[Image:Streaking prep. | + | |
+ | To steak yeast onto a plate you'll need: | ||
+ | |||
+ | * a flame source for sterilizing the inoculation needle | ||
+ | * an inoculation needle. A piece of wire works as well, but inoculation needles are cheap and you should pick one up when buying the petri dishes | ||
+ | * a sample of yeast. This could be an empty Wyeast smack pack, Whitle Labs vial, dregs from a bottle conditioned beer, an active fermentation, yeast sediment .... In this case I had an old White Labs vial into which I filled sterile wort to get the yeast fermenting again before taking a sample. | ||
+ | * an petri dish with malt agar that has been prepared in advance. (See [Making Plates Slants]) | ||
+ | |||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | [[Image:Streaking prep.jpg]] | ||
+ | |||
+ | <div style="clear:both;"></div> | ||
[[Image:Streaking sampling.jpg]] | [[Image:Streaking sampling.jpg]] | ||
+ | |||
+ | <div style="clear:both;"></div> | ||
[[Image:Streaking streaking.jpg]] | [[Image:Streaking streaking.jpg]] | ||
+ | |||
+ | <div style="clear:both;"></div> | ||
[[Image:Streaking grown plate.jpg]] | [[Image:Streaking grown plate.jpg]] |
Revision as of 03:03, 22 October 2008
What's needed
To steak yeast onto a plate you'll need:
- a flame source for sterilizing the inoculation needle
- an inoculation needle. A piece of wire works as well, but inoculation needles are cheap and you should pick one up when buying the petri dishes
- a sample of yeast. This could be an empty Wyeast smack pack, Whitle Labs vial, dregs from a bottle conditioned beer, an active fermentation, yeast sediment .... In this case I had an old White Labs vial into which I filled sterile wort to get the yeast fermenting again before taking a sample.
- an petri dish with malt agar that has been prepared in advance. (See [Making Plates Slants])