Difference between revisions of "Growing Yeast from a Plate"

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(What is needed?)
(Picking Colonies)
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=Picking Colonies=
 
=Picking Colonies=
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[[Image:Plate_w_colonies.jpg|right]]
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In order to ensure that you are only using yeast colonies that have grown from a single yeast cell only consider single round colonies of yeast. Yeas colonies are off-white with dull looking surface. Some of your streaks on the plate should have produces such colonies.

Revision as of 04:18, 3 March 2008

Growing yeast from a plate (petri dish) allows the brewer to select only a few single cell growth colonies. By doing so a pure culture can be obtained even if the starting culture was questionable. But because the number of yeast cells is very small during the first few steps, a sterile environment is necessary.

What is needed?

Needed for growing yeast.jpg
  • petri dish with yeast colonies (Yeast Cultures on Petri Dishes)
  • inoculation loop
  • flame source. Bunsen burner, alcohol lamp, blow torch or gas stove
  • vial with sterile starter wort

Picking Colonies

Plate w colonies.jpg

In order to ensure that you are only using yeast colonies that have grown from a single yeast cell only consider single round colonies of yeast. Yeas colonies are off-white with dull looking surface. Some of your streaks on the plate should have produces such colonies.